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human origin  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology human origin
    Human Origin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+origin/SDF-1%CE%B1/pm41731116-426-34-41
    Average 93 stars, based on 66 article reviews
    human origin - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Paving the Way for CCK2R-Targeted Peptide Receptor Radionuclide Therapy with [ 177 Lu]Lu-DOTA-MGS5 in Patients with Small Cell Lung Cancer.
    Article Snippet: .. Endogenous peroxidase activity was blocked, and sections were incubated with a mouse monoclonal CCK2R (E-3) antibody raised against amino acids 1–85 mapping at the N-terminus of CCK2R of human origin (sc-166690; Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:75 for 15 min at room temperature. .. The Bond Polymer Refine Detection Kit (DS9800; Leica Biosystems, Wetzlar, Germany) without the post-primary reagent was used for antibody detection, followed by DAB (3,3′-diaminobenzidine, BS04-500; Medac Diagnostica, Wedel, Germany) staining using brown chromogen (DS9800, Bond Polymer Refine Detection; Leica Biosystems, Wetzlar, Germany), hematoxylin counterstaining, dehydration, clearing and mounting using Pertex mounting medium (00801; Histolab, Goeteborg, Sweden).

    Article Title: Paving the Way for CCK2R-Targeted Peptide Receptor Radionuclide Therapy with [ 177 Lu]Lu-DOTA-MGS5 in Patients with Small Cell Lung Cancer
    Article Snippet: .. Endogenous peroxidase activity was blocked, and sections were incubated with a mouse monoclonal CCK2R (E-3) antibody raised against amino acids 1–85 mapping at the N-terminus of CCK2R of human origin (sc-166690; Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:75 for 15 min at room temperature. .. The Bond Polymer Refine Detection Kit (DS9800; Leica Biosystems, Wetzlar, Germany) without the post-primary reagent was used for antibody detection, followed by DAB (3,3′-diaminobenzidine, BS04-500; Medac Diagnostica, Wedel, Germany) staining using brown chromogen (DS9800, Bond Polymer Refine Detection; Leica Biosystems, Wetzlar, Germany), hematoxylin counterstaining, dehydration, clearing and mounting using Pertex mounting medium (00801; Histolab, Goeteborg, Sweden).

    Article Title: Peptide receptor radionuclide therapy targeting the cholecystokinin-2 receptor: Preclinical and first clinical experience in small cell lung cancer
    Article Snippet: .. Endogenous peroxidase activity was blocked, and sections were incubated with a mouse monoclonal CCK2R (E-3) antibody raised against amino acids 1-85 mapping at the N-terminus of CCK2R of human origin (sc-166690, Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:75 for 15 min at room temperature. .. Bond Polymer Refine Detection Kit (DS9800, Leica Biosystems, Wetzlar, Germany) without the post-primary reagent was used for antibody detection, followed by DAB (3,3’-diaminobenzidine, BS04-500, Medac Diagnostica, Wedel, Germany) staining using brown chromogen (DS9800, Bond Polymer Refine Detection, Leica Biosystems, Wetzlar, Germany), hematoxylin counterstaining, dehydration, clearing, and mounting using Pertex mounting medium (00801, Histolab, Goeteborg, Sweden).

    Incubation:

    Article Title: Paving the Way for CCK2R-Targeted Peptide Receptor Radionuclide Therapy with [ 177 Lu]Lu-DOTA-MGS5 in Patients with Small Cell Lung Cancer.
    Article Snippet: .. Endogenous peroxidase activity was blocked, and sections were incubated with a mouse monoclonal CCK2R (E-3) antibody raised against amino acids 1–85 mapping at the N-terminus of CCK2R of human origin (sc-166690; Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:75 for 15 min at room temperature. .. The Bond Polymer Refine Detection Kit (DS9800; Leica Biosystems, Wetzlar, Germany) without the post-primary reagent was used for antibody detection, followed by DAB (3,3′-diaminobenzidine, BS04-500; Medac Diagnostica, Wedel, Germany) staining using brown chromogen (DS9800, Bond Polymer Refine Detection; Leica Biosystems, Wetzlar, Germany), hematoxylin counterstaining, dehydration, clearing and mounting using Pertex mounting medium (00801; Histolab, Goeteborg, Sweden).

    Article Title: Paving the Way for CCK2R-Targeted Peptide Receptor Radionuclide Therapy with [ 177 Lu]Lu-DOTA-MGS5 in Patients with Small Cell Lung Cancer
    Article Snippet: .. Endogenous peroxidase activity was blocked, and sections were incubated with a mouse monoclonal CCK2R (E-3) antibody raised against amino acids 1–85 mapping at the N-terminus of CCK2R of human origin (sc-166690; Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:75 for 15 min at room temperature. .. The Bond Polymer Refine Detection Kit (DS9800; Leica Biosystems, Wetzlar, Germany) without the post-primary reagent was used for antibody detection, followed by DAB (3,3′-diaminobenzidine, BS04-500; Medac Diagnostica, Wedel, Germany) staining using brown chromogen (DS9800, Bond Polymer Refine Detection; Leica Biosystems, Wetzlar, Germany), hematoxylin counterstaining, dehydration, clearing and mounting using Pertex mounting medium (00801; Histolab, Goeteborg, Sweden).

    Article Title: Peptide receptor radionuclide therapy targeting the cholecystokinin-2 receptor: Preclinical and first clinical experience in small cell lung cancer
    Article Snippet: .. Endogenous peroxidase activity was blocked, and sections were incubated with a mouse monoclonal CCK2R (E-3) antibody raised against amino acids 1-85 mapping at the N-terminus of CCK2R of human origin (sc-166690, Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:75 for 15 min at room temperature. .. Bond Polymer Refine Detection Kit (DS9800, Leica Biosystems, Wetzlar, Germany) without the post-primary reagent was used for antibody detection, followed by DAB (3,3’-diaminobenzidine, BS04-500, Medac Diagnostica, Wedel, Germany) staining using brown chromogen (DS9800, Bond Polymer Refine Detection, Leica Biosystems, Wetzlar, Germany), hematoxylin counterstaining, dehydration, clearing, and mounting using Pertex mounting medium (00801, Histolab, Goeteborg, Sweden).

    Chromatin Immunoprecipitation:

    Article Title: Disorganized chromatin hierarchy and stem cell aging in a male patient of atypical laminopathy-based progeria mandibuloacral dysplasia type A
    Article Snippet: .. The lamin A/C antibody (Santa Cruz Biotechnology, sc376248) is specific for an epitope mapping between amino acids 2-29 at the N-terminus of Lamin A/C of human origin (https://www.scbt.com/p/lamin-a-c-antibody-e-1) and has been used for ChIP-seq assay in publications [PMID: 31316208 https://pubmed.ncbi.nlm.nih.gov/ 31316208/ and 32208162 https://pubmed.ncbi.nlm.nih.gov/32208162/]. ..

    Purification:

    Article Title: Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission.
    Article Snippet: .. Note that CXCL-12 or SDF-1a (hBA-68) purified protein is produced from Escherichia coli bacterial lysates (>98%) and supplied as 35-kDa biologically active, GST-tagged fusion protein corresponding to 68 amino acids of the SDF-1a of human origin (Catalog number sc-4654, obtained from Santa Cruz Biotechnologies Inc., USA). .. For cell scratch assay, HaCaT cells (1–3 × 1e6) or human keratinocyte primary cultures (5 × 1e5) were seeded in six/twelve-well plates with Dulbecco’s modified Eagle’s medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA).

    Article Title: Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission
    Article Snippet: .. Note that CXCL-12 or SDF-1a (hBA-68) purified protein is produced from Escherichia coli bacterial lysates (>98%) and supplied as 35-kDa biologically active, GST-tagged fusion protein corresponding to 68 amino acids of the SDF-1a of human origin (Catalog number sc-4654, obtained from Santa Cruz Biotechnologies Inc., USA). .. For cell scratch assay, HaCaT cells (1–3 × 1e6) or human keratinocyte primary cultures (5 × 1e5) were seeded in six/twelve-well plates with Dulbecco’s modified Eagle’s medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA).

    Produced:

    Article Title: Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission.
    Article Snippet: .. Note that CXCL-12 or SDF-1a (hBA-68) purified protein is produced from Escherichia coli bacterial lysates (>98%) and supplied as 35-kDa biologically active, GST-tagged fusion protein corresponding to 68 amino acids of the SDF-1a of human origin (Catalog number sc-4654, obtained from Santa Cruz Biotechnologies Inc., USA). .. For cell scratch assay, HaCaT cells (1–3 × 1e6) or human keratinocyte primary cultures (5 × 1e5) were seeded in six/twelve-well plates with Dulbecco’s modified Eagle’s medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA).

    Article Title: Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission
    Article Snippet: .. Note that CXCL-12 or SDF-1a (hBA-68) purified protein is produced from Escherichia coli bacterial lysates (>98%) and supplied as 35-kDa biologically active, GST-tagged fusion protein corresponding to 68 amino acids of the SDF-1a of human origin (Catalog number sc-4654, obtained from Santa Cruz Biotechnologies Inc., USA). .. For cell scratch assay, HaCaT cells (1–3 × 1e6) or human keratinocyte primary cultures (5 × 1e5) were seeded in six/twelve-well plates with Dulbecco’s modified Eagle’s medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA).



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